Jagoran Kimiyyar bazara: Lokacin da zafin zafin jiki na 40°C ya hadu da Gwajin Kwayoyin Halitta

Yanayin zafi ya ci gaba da kasancewa a yawancin China kwanan nan. A ranar 24 ga Yuli, Cibiyar Kula da Yanayi ta lardin Shandong ta ba da faɗakarwar zazzabi mai launin rawaya, tana hasashen yanayin yanayin “sauna-kamar” 35-37°C (111-133°F) da zafi 80% na kwanaki huɗu masu zuwa a cikin yankunan ƙasa. Yanayin zafi a wurare kamar Turpan, Xinjiang, yana gabatowa 48°C (111-133°F). Wuhan da Xiaogan, Hubei, suna ƙarƙashin faɗakarwar lemu, tare da yanayin zafi sama da 37 ° C a wasu yankuna. A cikin wannan zafi mai zafi, ƙananan ƙananan duniyar da ke ƙarƙashin saman pipettes suna fuskantar tartsatsi na ban mamaki - kwanciyar hankali na acid nucleic, ayyukan enzymes, da yanayin jiki na reagents duk suna cikin nutsuwa ta hanyar zafin rana.

Hakar acid nucleic ya zama tseren kan lokaci. Lokacin da zafin jiki na waje ya wuce 40 ° C, ko da tare da na'urar sanyaya iska a kunne, yawan zafin jiki na teburin aiki yakan yi sama da 28 ° C. A wannan lokacin, samfuran RNA sun bar a buɗe suna raguwa fiye da sau biyu cikin sauri kamar na bazara da kaka. A cikin hakar ƙwanƙwasa maganadisu, maganin buffer ɗin yana cika a cikin gida saboda haɓakar ƙarfin ƙarfi na ƙarfi, kuma lu'ulu'u suna haɗe cikin sauƙi. Waɗannan lu'ulu'u za su haifar da babban canji a cikin ingancin kama acid nucleic. Ƙaƙƙarfan ƙauyen kwayoyin halitta yana ƙaruwa lokaci guda. A 30 ° C, adadin chloroform volatilization yana ƙaruwa da 40% idan aka kwatanta da 25 ° C. Lokacin aiki, ya zama dole don tabbatar da cewa saurin iska a cikin hurumin hayaki shine 0.5m / s, kuma amfani da safofin hannu na nitrile don kiyaye tasirin kariya.

Gwaje-gwajen PCR suna fuskantar ƙarin rikice-rikicen yanayin zafi. Reagents kamar Taq enzyme da juyi transcriptase suna da matuƙar kula da jujjuyawar zafin jiki kwatsam. Kwangila akan bangon bututu bayan cirewa daga injin daskarewa -20 ° C na iya haifar da asarar ayyukan enzyme sama da 15% idan ya shiga tsarin amsawa. Har ila yau, maganin dNTP na iya nuna lalatawar da za a iya ganowa bayan kawai mintuna 5 na fallasa zuwa zafin daki (> 30°C). Har ila yau, yanayin zafi yana da cikas ga aikin kayan aiki. Lokacin da yanayin yanayin dakin gwaje-gwaje ya kasance> 35 ° C kuma ƙarancin zubar da zafi na kayan aikin PCR bai isa ba (<50 cm daga bango), bambancin zafin jiki na ciki zai iya kaiwa har zuwa 0.8 ° C. Wannan sabawa na iya haifar da haɓakar haɓakawa a gefen farantin rijiyar 96 zuwa ƙasa da sama da 40%. Yakamata a tsaftace matatun kura akai-akai (Turawar ƙurar yana rage tasirin zafi da kashi 50%), kuma yakamata a guji sanyaya iska kai tsaye. Bugu da ƙari, lokacin yin gwaje-gwajen PCR na dare, guje wa amfani da kayan aikin PCR a matsayin "firiji mai gyare-gyare" don adana samfurori. Ajiye a zafin jiki na 4°C na fiye da awanni 2 na iya haifar da kumburin ruwa bayan rufewar murfi mai zafi, yana lalata tsarin amsawa da yuwuwar lalata na'urorin ƙarfe na kayan aikin.

Fuskantar faɗakarwa mai tsayi mai tsayi, dakunan gwaje-gwajen kwayoyin yakamata su yi ƙararrawa. Yakamata a adana samfuran RNA masu daraja a bayan injin daskarewa -80°C, tare da iyakance damar zuwa lokacin zafi mai girma. Bude kofa na injin daskarewa -20°C fiye da sau biyar a rana zai ta'azzara canjin yanayin zafi. Kayan aiki mai zafi mai zafi yana buƙatar aƙalla 50 cm na sararin samaniyar zafi a bangarorin biyu da na baya. Bugu da ƙari, ana ba da shawarar sake fasalin lokacin gwaji: 7: 00-10: 00 AM don ayyuka masu zafin jiki kamar hakar RNA da qPCR lodi; 1: 00-4: 00 PM don aikin da ba na gwaji ba kamar nazarin bayanai. Wannan dabarar za ta iya hana kololuwar zafin jiki yadda ya kamata daga tsoma baki tare da matakai masu mahimmanci.

Gwajin kwayoyin halitta a lokacin zafin rana gwaji ne na fasaha da haƙuri. Ƙarƙashin rana ta rani da ba ta da ƙarfi, watakila lokaci ya yi da za ku ajiye pipette ɗinku kuma ku ƙara ƙarin akwati na kankara zuwa samfuran ku don ba da damar kayan aiki ya watsar da ƙarin zafi. Wannan girmamawa ga canjin yanayin zafi shine ainihin ingancin dakin gwaje-gwaje mafi daraja a lokacin lokacin rani mai zafi-bayan haka, a cikin zafin rana na 40 ° C, har ma da kwayoyin suna buƙatar “yankin polar wucin gadi” da aka kiyaye a hankali.