Nat med | Halin da Multi-Ops don tsara ƙwayar hadar da hade

Nat med | Tsarin da yawa don tsara ƙwayar da aka haɗa, ciki da kuma yanayin ƙasa na ƙwayar cuta na Colrisal Colrabiome tare da tsarin rigakafi
Kodayake na cikin cutar sankara na farko a cikin 'yan shekarun nan, jagororin asibiti na yau da kullun ko kuma rashin tsaro na DNA. Masu bincike sun lura da rashin daidaituwa tsakanin martanin shaidar kariya ta asali, da kuma buri mai tsauri a cikin ciwon kan cutar sankara da kuma tsira.

Kamar yadda bincike ya ci gaba, halayen cutar kansa ta asali, ciki har da yanayin yanayin cutar kansa, amma har yanzu akwai matsakaiciyar fahimta game da batun haƙuri.
Don watsa dangantakar da ke tsakanin rikice-rikice na phenotypic da sakamako, ƙungiyar masu bincike daga Cibiyar Kula da Kayayyakin Sidar kwanan nan ta haɗu da ingantattun ƙimar ƙwayoyin cuta (icr). Teamungiyar ta yi cikakken bincike game da sabbin kayan maye daga 348 tare da cutar sankara ta farko, cike da kayan kwalliya na rnana, da kuma daidaita sel mai kyau. An buga binciken a cikin maganin yanayi a matsayin "kumburi da aka haɗa, ciki, ciki da microbiome atolas na ciwon kai na mallaka".

Mataki na ashirin da aka buga a yanayi magani

Ac-IMAM

Researchers used an orthogonal genomic platform to analyze fresh frozen tumor samples and matched adjacent healthy colon tissue (tumor-normal pairs) from patients with a histologic diagnosis of colon cancer without systemic therapy. Dangane da duka-exome sequincing (Wes), ingancin ingancin bayanai, da hada mahaita da aka duba marasa bincike tare da masu bincike na ƙasa tare da bidian na shekaru 4.6. Kungiyar bincike mai suna wannan albarkatun Sidra-lumc AC-ICAM: taswira da jagora zuwa ma'amala ta rigakafi (Hoto 1).

Rarrabuwar kayan abinci ta amfani da iCR

Shiga wani abu mai amfani da kayan kwayoyin rigakafi na ci gaba da hana cutar kansa (ICR), Cutar cututtukan molanooma, mafitsara cutar melanoma, da cutar molanooma, da cutar kansa da cutar molanooma, da cutar kansa da cutar molanooma, da cutar molanooma, da cutar kansa da cutar molanooma, da cutar kansa da cutar molanooma, da cutar kansa da cutar molanooma, da cutar kansa da cutar molanooma, da cutar ta molanooma, da cutar kansa da cutar molanooma, da cutar molanooma, da cutar ta molanooma, da cutar kansa da nono. Hakanan ana samun alaƙa da amsa da rigakafin cututtukan daji, gami da cutar kansa.

Da farko, masu binciken sun tabbatar da sa hannu na ICM-ICAM, ta amfani da tsarin tantancewa na ICR. Masu bincike sun nuna haɓakar ƙwayar rigakafi da ke hade da subtypes na yarjejeniya (CMS), rarrabuwar kawuna ne na cutar kansa. Kungiyoyin CMS sun hada da CMS1 / CMS2 / Canonical, CMS3 / Metabolic da CMS4 / Meschymal. Binciken ya nuna cewa yawan bayanan ICR sun kasance amintattun hanyoyin sikila a cikin dukkan CMS na cutar CMS, da kuma hanyoyin ingantattun hanyoyin da suka shafi cmonsiuppresove da kuma strunsiupa da suka shafi cms4 kawai.

A cikin dukkan CMS, da yawa daga cikin kisan gilla (nk) da tobin sel da t subspeypes na rigakafi (Hoto na 1D), tabbatar da ingantaccen aikin iCR a cikin cutar kansa.

Hoto 1. Tsarin karatun AC-ICAM, rigakafi na rigakafi da ke da ƙarfi, sa hannu na Gene, rigakafi da kuma tsinkaye.
Icr kame tumo-wadatar, a fili, clonally clonally clonally clonally clonally clonally
Kawai 'yan tsiraru ne kawai na slls ba a ruwaito yawan ƙwayar cuta don tumo da yawa ba (ƙasa da 10%). Sabili da haka, yawancin ƙwayoyin na ciki suna magana a matsayin sel na ɓoye-ɓoye (sel na ɓoye). Matsakaicin daidaitawa tare da yawan sel sel na al'ada tare da tcrs mai sassauci da tcrs (an gano shi don kimanta sel sel (wanda za'a iya amfani da shi don kimanta slet sel (Hoto 2a). A cikin gungu na ICR (gaba da CMS daban-daban), mafi girman ikon silalid na rigakafi an lura da shi a cikin iCR-girma da kuma crs subtypepe cms1 / gungun sukari (Hoto na 2c), tare da mafi girman adadin ciwan-tummors. Using the whole transcriptome (18,270 genes), six ICR genes (IFNG, STAT1, IRF1, CCL5, GZMA, and CXCL10) were among the top ten genes positively associated with TCR immune SEQ clonality (Figure 2d). Immunoseq Tcr Clonilal da ƙarfi ya fi yawa yawan samrin fiye da groupara lura ta amfani da ƙwayar cuta - masu ba da izini CD8 + alamara (Hoto na 2f da 2g). A ƙarshe, bincike na sama yana nuna cewa sa hannu na iCR yana ɗaukar kasancewar ƙwayar cuta-wadatar kuma na iya bayyana abubuwan da ake tsammani.

Hoto na 2. Kwarewar TCR da daidaituwa tare da kwayoyin halittar da suka shafi rigakafi, subnu da subnetpulseps.
Abun Microbiome a cikin kyallen takarda masu lafiya da cutarwa
Masu binciken sun yi ta amfani da DNA RRN ta amfani da DNA wanda aka fitar daga matattarar ƙwayar cuta da ƙoshin lafiya daga marasa lafiya na 246 (adadi 3a). Don inganci, masu binciken sun bincika bayanai 16S RRNA RRNA Gent data daga ƙarin sabbin samfuran 42 waɗanda ba su dace da bincike ba. Da farko, masu binciken idan aka kwatanta da dangi da yawa daga cikin matched ciwan da nama. An kara da clostium cermerens a cikin ciwace-ciwacen cuta idan aka kwatanta da kyawawan samfuran lafiya (Hoto na 3a-3d). Babu wani banbanci mai mahimmanci a cikin bambancin Alfa (bambancin halitta da yawa a cikin samfurin guda ɗaya) tsakanin ƙuruciya mai kyau, kuma ragi mai sauki a cikin iCR-ƙananan ciwan jini.
Don gano ƙungiyoyi masu dacewa tsakanin bayanan bayanan microbial da sakamakon binciken da suka yi niyya don amfani da kayan aikin na RRRiome waɗanda ke yin hasashen fasalin microbiome da ke haifar da rayuwa. A AC-ICAAM246, masu binciken sun gudu da samfurin OS Cox wanda aka zaɓa guda 41 tare da haɗarin rashin daidaituwa (hade da bambancin mace-mace (hade da bambancin mace-mace (Hoto na 3F).
A cikin wannan horarwar cohort (Icam246), ƙarancin MBR (MBB <0, ƙananan mbr) an danganta shi da ƙarancin haɗarin mutuwa (kashi 85%). Masu bincike sun tabbatar da ƙungiyar tsakanin ƙananan MBB (haɗarin) da tsawan OS a cikin kayan haɗin gwiwar da suka dace (ICAM42 da TCGA-Coad). (Hoto na 3) Binciken ya nuna daidaituwa mai ƙarfi tsakanin cocci da MBB, waɗanda suke kama da ƙari da nama da ƙoshin lafiya.

Hoto na 3. Microbiome a cikin tumo da kyallen takarda da kuma dangantakar da ke da icr da kuma rayuwa mai haƙuri.
Ƙarshe
An yi amfani da tsarin binciken da yawa a cikin wannan binciken yana ba da cikakken bincike da kuma nazarin sa hannu na rigakafin amsar da cutar kansa da tsarin rigakafi. Deep Sequin na kumburi da kyamai masu lafiya sun bayyana cewa tasirin tasirin icr na iya zama saboda iyawarsa na iya ɗaukar tsiro-takamaiman da kuma wataƙila tayin antigen.

Ta hanyar nazarin damuwa na ƙwayar cuta ta amfani da kayan aikin 16s RRNA RRAAM, ƙungiyar haɗarin haɗi) tare da ƙimar microbiome) tare da ƙimar ƙwayoyin cuta. Kodayake an samo wannan sa hannu daga samfurori masu ƙarfi, akwai wani abu mai ƙarfi tsakanin ƙoshin lafiya na lafiya, yana ba da shawara cewa wannan sa hannu na iya ɗaukar gut microboome na marasa lafiya. Ta hanyar hada ICR da MBB, yana yiwuwa a gano da tabbatar da ɗalibin ɗalibin opcic wanda ke annabta rayuwa a cikin marasa lafiya da cutar kansa. Ditarancin binciken da yawa yana samar da hanya don mafi kyawun fahimtar ilimin cutar kansa da taimako gano keɓaɓɓun hanyoyin da ke gabatowa.

Tunani:
Roelands, J., kuppen, ahm, Ahmed, ei et al. Kwayar hade da hade, cikin nutsuwa da microbiome atlas na ciwon kai. Nat Med Med, 1273-1286 (2023).