Gwajin DNA METHyLy

A farkon gano cutar kansa dangane da ruwa ruwa shine sabon shugabanci na gano cutar kansa, tare da manufar cutar kansa na kasar nan, tare da manufar gano cutar kansa ko kuma cutar cututtukan farko. An yi amfani da shi sosai azaman mai ilimin cutar ƙwayar cuta don ganewar asali daban-daban, gami da cutar sankara, ciwan mahaifa, gastrointestinal na jijiya.

Samuwar dandamali don gano shimfidar wuri (methylscape) masu yuwuwar inganta ra'ayoyi da wuri, sa marasa lafiya a farkon sakamakon yarjejeniyar.

Kwanan nan, masu bincike sun kirkiro dandamali mai sauki da kai tsaye don gano abubuwan da ke tattare da kayan tarihi na kayan tarihi wanda ke ba da damar da farkon zane na ciwace-ciwacen daji. Za'a iya aiwatar da allo da wuri don cutar seukemia a cikin mintina 15 bayan hakar DNA daga samfurin jini, tare da daidaito na 90.0%. Taken hanya yana da saurin gano cutar kansa a cikin jini ta amfani da Aunpe-conteline-congard Aunps da kuma wayar tarho mai ba da damar sarrafa injiniyan.

Hoto 1

An nuna wannan a cikin Hoto na 1. Da farko dai, ana amfani da maganin ruwa na ruwa don soke gutsutsuren DNA. An kara da CYST / AUNSPS zuwa ga m gauraye. Na al'ada da metgant dna suna da kayan methyanes daban-daban, sakamakon haifar da gutsattsarin DNA da nau'ikan ƙirar kai. DNA ta gama gari kuma a ƙarshe tara Cyst / AUNSPS, wanda ke haifar da yanayin jan hankali na CYST / AUNSPS, saboda canji a cikin shuɗi zuwa shuɗi mai shuɗi. Sabanin haka, bayanin martaba na Methyration na Ciwon Cancery DNA yana haifar da samar da manyan gunguna na DNA.

Hotunan faranti na 96-da kyau suna amfani da kyamarar ta wayar hannu. Ciwon daji ya auna ta hanyar wayoyin salula tare da koyon injin idan aka kwatanta da hanyoyin da ke faruwa.

Nunin cutar kansa a cikin samfuran jini na gaske

Don haɓaka amfanin dandalin magunguna, masu binciken da aka sanya su firikwensin da suka samu nasarar rarrabe tsakanin su na yau da kullun a cikin samfuran jini. Tsarin methyly a shafin CPG sites Epigeetetically daidaita ma'anar Gene. A kusan duk nau'ikan cutar kansa, canje-canje a cikin DNA methyation don haka a cikin bayyana kwayoyin halittar da ke inganta Tumurogensis don madadin.

A matsayinmu na wasu cututtukan daji da ke hade da DNA MOWNLASS, masu binciken sunyi amfani da samfuran jini daga marasa lafiya na Methemiya da kuma ikon yin bincike game da tasirin methylat. Wannan methylation landscape biomarker ba wai kawai outperfors dake allon allon hanyar da ke da sauri ba, har ma yana nuna yiwuwar mirgine na farkon faduwa ta amfani da wannan sauki da madaidaiciya Assay.

DNA daga samfurori na jini daga marasa lafiya 31 na wajibi da mutane 12 masu lafiya na likita. Kamar yadda aka nuna a cikin akwatin makirci a cikin Hoto na 2a, dangi na dangin cutar kansar (δE650/550) ya kasance ƙasa da na DNA daga samfurori na yau da kullun. Wannan ya gana ne saboda inganta haɓakar hydrophobiwricity na hydrophobiity ya kai ga m hadgilice na cutar kansa na ciki, wanda ya hana tardar Cyst / Aunps. A sakamakon haka, waɗannan abubuwan nanopartich sun tarwatsa gaba daya a cikin tarin abubuwan da cutar kansa, wanda ya haifar da wani watsawa daban-daban na CYST / DNA DNA ta al'ada ce. An samo su ta hanyar bambance bambancen daga ƙimar ΔA650 / 525 zuwa matsakaicin darajar.

Hoto na 2. (A) Darajojin wasan kwaikwayo na wasan kwaikwayo na CYST / AUNSPS mafita da ke nuna kasancewar al'ada (shuɗi) da cutar kansa (jan) a cikin ingantaccen yanayi

(Da650 / 525) na kayan kwalliya; (b) Binciken ROC da kimantawa na gwaje-gwajen bincike. (C) Rikicewar matrix don ganewar asali na al'ada da cutar kansa. (d) Senditoci, takamaiman darajar tsinkaya (ppv), ƙimar tsinkaya mara kyau (NPV) da daidaito na hanyar haɓaka.

Kamar yadda aka nuna a cikin Hoto na 2B, yankin a karkashin roc curve (Auc = 0.9274) da aka samu don haɓaka mai haɓaka ya nuna babban abin kula da tsari. Kamar yadda za a iya gani daga akwatin mãkirci, mafi ƙasƙantar da mafi ƙasƙanci wanda ke wakiltar ƙungiyar DNA na yau da kullun ba su da nutsuwa daga mafi girman ma'anar wakiltar cutar kansar cutar ta cikin cutar ta cutar ta Cewa. Saboda haka, ana amfani da juyin juya hali don bambancewa tsakanin kungiyoyin al'ada da cututtukan daji. Ganin sahun masu canji masu zaman kansu, yana kimanta yiwuwar wani taron da ke faruwa, kamar shi ne ciwon kansa. Dogaro mai dogaro tsakanin 0 da 1. Sakamakon shine yiwuwar hakan. Mun yanke shawarar bayyanar cutar kansa (P) dangane da δa650/550 / 525 kamar haka.

Inda B = 5.3533, W1 = -6.965. Don rarrabuwa samfurin, yiwuwar ƙasa da 0.5 yana nuna samfurin al'ada, yayin da yuwuwar 0.5 ko sama yana nuna samfurin cutar kansa. Hoto na 2CC ya nuna rikice rikice da matrix da aka samar da shi daga barin wurin da ya faru, wanda aka yi amfani da shi don tabbatar da kwanciyar hankali na hanyar rarrabuwa. Hoto na 2D taƙaice kimar gwajin bincike na hanyar, gami da hankali, takamaiman, ƙimar tsinkaya (PPV) da ƙimar hangen nesa (NPV).

Masu samar da salula na wayo

Don kara sauƙaƙe gwajin samfurin ba tare da amfani da Spectrophotereter ba, masu binciken sunyi amfani da bayanan sirri (AI) don fassara launi na maganin da kuma rarrabewa tsakanin mutane na yau da kullun da kuma masu haihuwa. Ba da wannan, ana amfani da hangen nemin bayanin kwamfuta don fassara launi na Cyst / AUNPS ta hanyar da aka ɗauka ta amfani da faranti na 96-da aka ɗauka ta hanyar kyamarar wayar tarho. Invententarni na wucin gadi na iya rage farashi da haɓaka haɗi a fassarar launi na Nanoparticle. A ƙarshe, ƙirar koyo na koyan inji, gami da gandun daji na bazuwar (rf) da kuma tallafin injin vector (SVM) da aka horar don gina ƙirar. Dukkanin samfuran RF da SVM sun rarrabe samfurori masu kyau da mara kyau tare da daidaito na 90.0%. Wannan yana nuna cewa amfani da kayan wucin gadi a cikin wayar hannu ta hanyar wayar salula mai yiwuwa ne.

Hoto 3. (a) Matsayin aji na mafita da aka yi rikodin yayin shirye-shiryen samfurin don matakan siyarwar hoto. (b) Misali hoto da aka ɗauka yayin yanayin siye da hoto. (C) Canjin launi na CYST / AUNSPS mafita a kowane rijiyar farantin 96 da aka fitar daga hoton (b).

Ta amfani da Cyst / AUNPS, masu bincike sun sami nasarar samar da dandamali mai sauƙin bincike kan ganowa na Metwalle na yau da kullun yayin amfani da samfurori na ainihi don gwajin fata. Maƙwakin firikwensin ya nuna cewa DNA Extorces daga ainihin samfuran jini ya sami damar da yawa na cutar sankarau a cikin mintina 15, kuma ya nuna daidaito na 95.3%. Don kara sauƙaƙewa gwaji na gwaji ta hanyar kawar da buƙatar mafita, na masu-kyau na amfani da hoto na yau da kullun, kuma daidaito ma an sami damar zuwa 90.0%.

Tunani: Doi: 10.1039 / d2ra05725e