Labarai - Kayan Tsarkakewa na Takardar Shafawa ta Oral na Bigfish FeiXu: Mafita Mai Inganci Ga Cirewar DNA Daga Samfuran Baki

A cikin fannoninBinciken asibiti a cikin vitro (IVD), nau'in halittar jini, da binciken kwayoyin halitta, samfuran baki - kamarshafawa ta baki, shafawa ta makogwaro, da kuma yau- ana amfani da su sosai don gwajin nucleic acid sabodaTarawa mai sauƙi, yanayi mara haɗari, da kuma tsarin ɗaukar samfur mara zafiDuk da haka, samfuran da ake sha ta baki galibi suna ɗauke daiyakataccen adadin nucleic acidkuma galibi suna gurbata dafurotin da sauran ƙazantaHanyoyin cirewa na gargajiya galibi suna fama da suhadaddun hanyoyin aiki, ƙarancin inganci, da kuma amfani da magungunan guba, wanda zai iya yin illa sosai ga daidaito da kwanciyar hankali na aikace-aikacen da ke ƙasa kamarPCR/qPCR da tsarin tsara na gaba (NGS).

TheKayan Aikin Hana Jinin Halittar DNA na BFMP06 Mai Amfani da Bead Mai Magana, wanda aka haɓaka taFasahar Halittar Hangzhou Bigfish FeiXu, yana bayar damafita mai aminci, inganci, kuma abin dogarodon fitar da samfurin DNA na baki. Tare da ƙirar fasaha mai ƙirƙira da ƙa'idodin aiki masu tsauri, wannan kayan aikin ya zama kayan aiki amintacce ga dakunan gwaje-gwaje na asibiti da na bincike.

An gina kayan aikin BFMP06 ne a kan wani tsari mai kama da na'urar zamani.tsarin buffer da aka inganta musammantare daƘwayoyin maganadisu na musamman na hydroxyl na DNA, yana samar da ingantaccen aikin tsarkake sinadarin nucleic acid. Bayan an saka samfurin a cikin ma'aunin lysis, ana wargaza sassan ƙwayoyin halitta kuma ana sakin ƙwayoyin nucleic acid. Ƙungiyoyin aiki a saman beads na maganadisu suna ɗaure DNA kyauta, suna samar da tsayayyen tsari.hadaddun ƙwayoyin halitta na magnetic-DNA.

A ƙarƙashin filin maganadisu na waje, hadaddun suna shigamatakai biyu na wanke-wanke daidaidon cire sunadarai, gishiri, da sauran gurɓatattun abubuwa gaba ɗaya. A ƙarshe,DNA mai tsarki sosaiAna cire shi ta hanyar amfani da ma'aunin ruwa mai ƙarfi (elition buffer).

Bayanin Samfurin

Wannan samfurin yana amfani da tsarin buffer na musamman wanda aka haɓaka kuma aka inganta shi tare dabeads na magnetic waɗanda ke ɗaure DNA musamman, yana ba da damar sha, rabuwa, da tsarkake ƙwayoyin nucleic acid cikin sauri. Ya dace sosai daWarewa cikin sauri da inganci na DNA na kwayoyin halitta daga swabs na baki, swabs na makogwaro, da samfuran yau, yayin da yake cire sauran sunadaran da gishiri yadda ya kamata.

Lokacin amfani da shi tare daKayan aikin fitar da sinadarin nucleic acid mai tushen beads na Bigfish FeiXu, kayan aikin ya dace dababban fitarwa ta atomatikDNA ɗin kwayar halittar da aka tsarkake yana datsarki mai girma da kuma kyakkyawan inganci, wanda hakan ya dace da aikace-aikace iri-iri na ƙasa, ciki har daPCR/qPCR da NGS.

Fasallolin Samfura

Babban Inganci

Yana ware kuma yana tsarkake DNA na kwayoyin halitta dagashafawa ta baki, shafawa ta makogwaro, da kuma yau, isar dayawan amfanin ƙasa mai yawa da kuma tsarki mai girma.

Da sauri da kuma dacewa

Ba a buƙatar maimaita aikin tace iska ko injin tacewa. Ya dace da kayan aikin cirewa ta atomatik, wanda hakan ya sa ya dace damanyan samfuran sarrafawa.

Lafiya kuma Ba Mai Guba Ba

Babu buƙatar magungunan ƙwayoyin cuta masu guba kamar suphenol ko chloroform.

Kayan aiki masu jituwa

Bigfish FeiXu BFEX-16E

BFEX-32

BFEX-32E

BFEX-96

Sakamakon Gwaji

Samfuran swab na baki (an nutsar da su a cikinMaganin kiyayewa 400 μL) da samfuran yau (Maganin kiyayewa na 200 μL da 200 μLan sarrafa su ta amfani daKayan tsarkake DNA na Bigfish FeiXuAn cire DNA a cikin70 μL ma'aunin fitarwakuma an yi nazari taElectrophoresis na gel agarose, kamar yadda aka nuna a cikin hoton da ke ƙasa.