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Hanyar Magnetic Bead Ta Magnetic Ta Magance Kalubalen Cire DNA Daga Ruwa Mai Muhalli Yadda Ya Kamata
A fannoni kamar binciken ƙwayoyin cuta na muhalli da kuma sa ido kan gurɓatar ruwa, cire DNA mai inganci muhimmin abu ne ga aikace-aikacen da ke ƙasa, ciki har da PCR/qPCR da tsarin tsara yanayi na gaba (NGS). Duk da haka, samfuran ruwan muhalli suna da matuƙar rikitarwa, suna ɗauke da al'ummomin ƙwayoyin cuta daban-daban, nau'ikan ƙwayoyin cuta masu wahalar yin amfani da su kamar ƙwayoyin cuta masu kyau na Gram, da kuma ƙalubalen da suka daɗe suna da alaƙa da hanyoyin cirewa na gargajiya - kamar amfani da sinadarai masu guba da hanyoyin da suka rikitar da masu bincike - waɗanda ke ci gaba da damun masu bincike.
Yanzu haka, Bigfish Sequencing ta gabatar da kayan aikin cirewa da tsarkakewa na DNA na Genomic da ke tushen ruwa mai suna BFMP24R Magnetic Bead, wanda ke samar da cikakkiyar mafita ga waɗannan ƙalubalen ta hanyar fasahar zamani da ƙira mai sauƙin amfani.
Bayanin Samfuri
Wannan kayan aikin ya dogara ne akan ingantaccen tsarin ma'ajiyar bayanai tare da beads na nano magnetic masu aiki mai kyau. DNA na genomic yana ɗaure musamman ga ƙungiyoyi masu aiki akan saman bead kuma an raba shi a ƙarƙashin filin maganadisu na waje. Bayan matakai da yawa na wankewa a hankali don cire sunadarai, gishiri, da sauran ƙazanta, a ƙarshe an cire DNA na genomic mai tsarki.
An tsara shi musamman don samfuran ruwan muhalli, kayan aikin yana fitar da DNA na ƙwayoyin cuta da aka tattara a kan membranes na tacewa cikin inganci, gami da ƙwayoyin cuta na Gram-negative da Gram-positive (har zuwa ƙwayoyin cuta 2 × 10⁹ a kowace membrane ɗaya na tacewa). Yana dacewa da tsarin cire sinadarin nucleic acid mai sarrafa kansa don sarrafa yawan aiki. DNA ɗin da aka cire yana da inganci iri ɗaya kuma ana iya amfani da shi kai tsaye don PCR/qPCR, NGS, da sauran aikace-aikacen da ke ƙasa.
Fasallolin Samfura
1. Ƙarfin Cire Kwayoyin cuta Mai Faɗi
Yana cire ƙwayoyin cuta na Gram-negative da Gram-positive yadda ya kamata daga samfuran ruwa, yana rufe al'ummomin ƙwayoyin cuta da aka fi samu a cikin ruwan sha da muhallin ruwa, kuma yana biyan buƙatun nazari daban-daban.
2. Tsarkakakken Tsafta da Yawan Yawa
Yana samar da DNA mai tsarki, ba tare da gurɓatattun abubuwa masu hana shi ba, da kuma wadataccen amfani mai kyau wanda ya dace da amfani da kwayoyin halitta kai tsaye.
3. Dacewa da Aiki ta atomatik da kuma Ingantaccen Aiki
Cikakken jituwa da tsarin fitar da sinadarin nucleic acid ta atomatik na Bigfish, yana tallafawa sarrafa samfura 32 ko 96 a lokaci guda, yana rage lokacin sarrafawa sosai da inganta ingancin dakin gwaje-gwaje.
4. Aiki mai aminci da sauƙin amfani
Ba a buƙatar sinadaran sinadarai masu guba kamar phenol ko chloroform, wanda hakan ke rage haɗarin amincin dakin gwaje-gwaje. Ana sanya sinadaran a cikin faranti 96 na rijiyoyi, wanda ke rage kurakuran bututun da hannu da kuma sauƙaƙe ayyukan aiki.
Kayan aiki masu jituwa
Bigfish BFEX-16E
BFEX-32
BFEX-32E
BFEX-96E
Sakamakon Gwaji
An tace samfurin ruwan kogi mai nauyin milimita 600 ta cikin wani membrane, sannan aka cire DNA ta amfani da kayan aikin cirewa da tsarkake DNA na ruwa mai kama da Bigfish tare da kayan aikin da suka dace. Daga baya an yi nazarin DNA ɗin da aka cire ta hanyar amfani da agarose gel electrophoresis.
M: Alama1, 2: Samfuran ruwan kogi
Bayanin Samfura
Lokacin Saƙo: Disamba-18-2025
Saitunan Sirri