2 × SYBR kore qPCR MIX (Tare da babban ROX)

Siffofin samfurin

Wannan samfurin, 2×SYBR kore qPCR MIX, yana zuwa a cikin bututu ɗaya wanda ya ƙunshi dukkan abubuwan da ake buƙata don haɓaka PCR da gano su, gami da Taq DNA poLymerase, SYBR kore I dye, High ROX Reference Dye, dNTPs, Mg2+, da PCR buffer.
Rini na kore na SYBR I rini ne mai launin kore wanda ke ɗaure da DNA mai launin biyu (DNA mai launin biyu, dsDNA) yankin ƙaramin ramin helix biyu. SYBR kore na I yana haskakawa da rauni a cikin yanayin 'yanci, amma da zarar ya ɗaure da DNA mai launin biyu, haskensa yana ƙaruwa sosai. Wannan yana ba da damar ƙididdige adadin DNA mai launin biyu da aka samar yayin haɓaka PCR ta hanyar gano ƙarfin hasken.
Ana amfani da ROX a matsayin rini na gyara don gyara canjin hasken da ba shi da alaƙa da PCR, don haka rage bambance-bambancen sarari. Irin waɗannan bambance-bambancen na iya faruwa ne ta hanyar dalilai daban-daban, kamar kuskuren pipette ko ƙafewar samfurin. Kayan aikin auna hasken da ba su da haske daban-daban suna da buƙatu daban-daban ga ROX, kuma wannan samfurin ya dace da masu nazarin ƙididdigar hasken da ke buƙatar gyara mai girma na ROX.