2 × SYBR kore qPCR MIX (Tare da Babban ROX)

Siffofin samfur

Wannan samfurin, 2 × SYBR kore qPCR MIX, ya zo a cikin bututu guda ɗaya wanda ke ɗauke da duk abubuwan da ake buƙata don haɓakawa da gano PCR, gami da Taq DNA poLymerase, SYBR kore I dye, Babban ROX Reference Dye, dNTPs, Mg2+, da PCR buffer.
SYBR kore na rini ne koren kyalli wanda ke ɗaure da DNA mai ɗaure biyu (DNA guda biyu, dsDNA) ƙaramin yanki na helix biyu. , haskensa yana haɓaka sosai. Wannan yana ba da damar ƙididdige adadin DNA mai ɗaure biyu da aka samar yayin haɓaka PCR ta gano ƙarfin haske.
Ana amfani da ROX azaman rini na gyare-gyare don gyara sauyin haske maras alaƙa da PCR, don haka rage bambance-bambancen sarari. Irin waɗannan bambance-bambance na iya haifar da abubuwa daban-daban, irin su kuskuren pipette ko fitar da samfurin. Na'urorin ƙididdige haske daban-daban suna da buƙatu daban-daban don ROX, kuma wannan samfurin ya dace da masu nazarin ƙididdige hasken haske waɗanda ke buƙatar gyaran ROX mai girma.